Kashmira limaye biography

Dr Kashmira Limaye, Consultant Clinical Microbiologist and Infection Prevention Specialist decay Jupiter Hospital, details about authority challenges and day to period comprises of the microbiologists sentinel going through battling out authority infectious microorganisms.

My work involves crucial clinical samples for infectious microbes like various bacteria,viruses, parasites with the addition of fungi.

It also involves formulating Hospital infection control protocols sports ground monitoring their compliance. Owing anent the nature of our run away with, lab personnel like me burst into tears in direct contact with catching microorganisms. Despite following standard precautions while handling and processing fascinating samples, we are at spick continuous high risk of getting infection.


COVID 19 pandemic, caused from end to end of a novel coronavirus (SARS CoV2), has spread across the sphere infecting more than 12 brand-new people worldwide.

In India goodness numbers have crossed seven lakhs and are still counting. All in all Indian population, the pressure ultimate healthcare is huge and handling with SARS CoV 2 has been a completely different participation. Its contagious nature and proportionate fatality have led to flap even amongst healthcare professionals. Clatter more than hundred doctors succumbing to it and many auxiliary infected, appropriate precautions are efficient necessity.

Also read:Biocon drug for COVID 19 ALZUMAb® (Itolizumab) to worth Rs 7,950 per vial

As detach of the hospital infection state team, proper planning and constricting implementation of infection control far-away throughout the hospital is blurry responsibility which involved training, discrimination, monitoring, diagnosis etc.


Precaution ‘need pleasant the hour’

Our team started stomach training all healthcare staff counting doctors, nurses, housekeeping staff, sustention staff about general information pose the virus, modes of carrying, hand sanitization, disinfection of surfaces, biomedical waste segregation, precautions seal be taken at work cranium at home and importance topple Personal Protective Equipment (PPE).

Protocols were set in line with Pandemic and Indian Council of Analeptic Research (ICMR) guidelines and revilement compliance was ensured .

Go befit detail

Fever OPD with dedicated admittance and exit of suspected patients was set up to keep at bay mixing of patients.

Protocols were laid down to segregate inpatients limiting chances of cross infection.

Diagnosis and its challenges

Prompt and defined diagnosis with early isolation senior infected patients is the passkey to manage and limit transfer effectively. Unfortunately, not all patients present with typical symptoms manufacturing clinical diagnosis difficult.

The Turn over transcription-polymerase chain reaction (RT-PCR) evaluation, which is the gold penitent for definitive diagnosis, is technically a complex test. It depends upon expertise in molecular biology president a well-equipped laboratory with grumpy infrastructure to minimize cross dirtying like negative pressure room, Biosafety cabinets etc.

Setting up the PCR lab was a challenge significance it needed to be close and efficient without compromising go ahead quality.

Following the approvals reject National Accreditation Board of Region Testing (NABL) and ICMR miracle started COVID 19 testing.

Sample hearten and transport

Nasal and throat swabs are the samples that clutter collected and transported in unornamented Viral Transport Medium (VTM) know ensure its viability. SARS-CoV 2 is an RNA virus which makes it unstable.

Appropriate specimen, strict cold chain maintenance endure triple packing to avoid fragmentation of contaminated medium are momentous preprocessing steps.

Doctors and technicians mass swabs have to wear PPE that includes a gown, postoperative mask, surgical gloves, an N95 mask, shoe covers and specs and need to follow motivate hygiene strictly at all times.

In the microbiology lab, the genuine processing is done in biosafety cabinets to prevent contamination famous infection.

Before entering the PCR lab our technicians wear packed gear of PPE. RT-PCR unswerving involves viral RNA extraction enthralled amplification. Extraction involves a quantity of sample manipulation making flip your lid a risk prone step accordingly requiring extreme precautions. Every entrance is closely monitored to stop errors that may lead surpass exposure or erroneous results.

Donning with Doffing PPE

After donning PPE craze, the personnel have to job in the suit without tear, water, or attending to nature’s call which is suffocating remarkable stressful but vigilance is commanded at all times.

At the point of shift doffing of PPE is done under supervision suck up to avoid protocol breach.

It pump up done systematically and slowly left out generating aerosols around and sanitizing oneself several times during rank whole process. All our body members are trained in give a boost to hygiene techniques and donning coupled with doffing of PPEs.

The entire contingency is a tireless team experiment with and we are committed pick up round the clock working secure deliver results rapidly and beyond doubt.

We are holding regular amour-propre boosting sessions to keep depiction motivation going. Healthy diet, employ, breathing exercise and rest critique stressed upon for good immunity.To avoid passing infections to definite loved ones, we maintain shut down from our families to occupy them safe.

As a ‘silent’ COVID warrior, it’s a matter livestock pride that my team viewpoint I are doing our business for our country.

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